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| Preferred Samples for
DNA Testing |
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| BUCCAL SWAB SAMPLES ARE THE IDEAL FOR DNA TESTING |
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Buccal swab |
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Blood (liquid or on gauze or on swab) |
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Eye brows (3-5) |
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Hairs (3-5) with roots or follicles |
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Cigarette butts (3-5) |
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| The following table shows the possible sources for DNA testing. |
| Sample type |
Total samples tested |
Positive results |
Success rate (%) |
| Muscle tissue |
2 |
2 |
100 |
| Buccal swab |
3019 |
2926 |
97 |
| Liquid blood |
38 |
36 |
95 |
| Blood swab |
173 |
162 |
94 |
| Slides (without cover glasses and dyes) |
10 |
9 |
90 |
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| Forensic samples |
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| Eye brows (3-5) |
15 |
14 |
93 |
| Rolled cigarettes (joints) |
72 |
65 |
90 |
| Blood on various surfaces |
4993 |
4326 |
87 |
| Saliva on paper |
31 |
26 |
84 |
| Hairs (3-5) with roots |
15 |
12 |
80 |
| Umbilical cord (fresh, lest than 1 year old) |
10 |
8 |
80 |
| Umbilical cord (old, greater than 1 year) |
6 |
3 |
50 |
| Cigarette butts |
1494 |
1073 |
72 |
| Buccal swab |
182 |
125 |
69 |
| Chewing gum |
22 |
12 |
55 |
| Toothbrush |
4 |
2 |
50 |
| Cigars |
2 |
1 |
50 |
| Ear wax |
8 |
4 |
50 |
| Saliva on bottles, soda cans (lips touch on bottle or can) |
49 |
18 |
37 |
| Saliva on a ski mask |
3 |
1 |
33 |
| Biological remains |
2304 |
742 |
32 |
| Nails |
10 |
2 |
20 |
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| Miscellaneous samples |
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| Underwear |
50 |
25 |
50 |
| Diapers |
4 |
2 |
50 |
| Tampon |
30 |
15 |
50 |
| Sweaty clothing (unwashed) |
10 |
1 |
10 |
| Shoe laces |
10 |
1 |
10 |
| Eye glasses (sun glasses or prescription glasses) |
20 |
1 |
5 |
| Any other sources could be tested |
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| Notes: |
| There are a number of factors that play a role in the ability to obtain a genetic profile, such as: |
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Quantity, the smaller the sample, the less DNA that may be obtained. |
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Environmental damage, chemical exposure, humidity, all play a role in DNA damage, which all in various ways affect the amount of recoverable and useful DNA. |
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Contaminants, there are a number of chemicals that can affect the success rate of the reactions involved in the process of obtaining a profile, such us excess dyes, oils, colorants, etc that may be present on some samples. |
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Hair samples work better, if they are freshly plucked, as opposed to fallen off hairs. Also adult hair samples work better, as opposed to infant hair samples. As the roots are pretty small and would require greater number of hairs. |
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In summary, whereas we desire samples that we know have a high success rate (of obtaining a profile), we will work with whatever samples are available and will do our best, understanding the variability in obtaining a profile is dependent on the nature and quality of each individual sample. |
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| Some of the data were gathered from Promega Corporation research team (www.promega.com). |
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