Plant DNA Testing

Plant DNA Testing

The Plant DNA C-values Database is a comprehensive catalogue of C-value (nuclear DNA content, or in diploids, genome size) data for land plants and algae.

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Plant DNA Database
Plant DNA Database
The database was originally launched as the "Angiosperm DNA C-values Database" in April 1997, essentially as an online version of collected data lists that had been published by Prof. Bennett and colleagues since the 1970s. Release 1.0 of the more inclusive Plant DNA C-values Database was launched in 2001, with subsequent releases 2.0 in January 2003 and 3.0 in December 2004. In addition to the angiosperm dataset made available in 1997, the database has been expanded taxonomically several times and now includes data from pteridophytes (since 2000), gymnosperms (since 2001), bryophytes (since 2001), and algae.
Plant DNA Extraction
Plant DNA Extraction
Thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibits restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants.
Ribonucleic Acid
Ribonucleic Acid (RNA) plays a pivotal role in 'one gene - one protein' theorem central to biology. Messenger RNA (mRNA) is synthesized as a sort of portable version of the gene encoded in the DNA. In eukaryotic cells it travels from the nucleus, where the DNA is located, into the cytoplasm, where the ribosome’s or protein-making machinery is located. In both eukaryotes and prokaryotes mRNA is the gene copy used by the ribosomes in the synthesis of the protein itself.
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