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DNA extraction is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic steps in a DNA extraction, the details of which may vary depending on the type of sample and any substances that may interfere with the extraction and subsequent analysis. First, you need to find something that contains DNA. Since DNA is the blueprint for life, everything living contains DNA.
Our staff works hard to make the DNA Identity Testing Center your choice of provider for all DNA testing services. We ensure superior quality, service and convenience to our clients. In our commitment to providing convenience, please know that we provide a free DNASwabTM Home DNA Collection Kit for use in all types of private DNA tests.
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Detecting DNA
A diphenylamine (DPA) indicators will confirm the presence of DNA. This procedure involves chemical hydrolysis of DNA: when heated (e.g. =95oC) in acid, the reaction requires a deoxyribose sugar and therefore is specific for DNA. Under these conditions, the 2-deoxyribose is converted to w-hydroxylevulinyl aldehyde, which reacts with the compound, diphenylamine, to produce a blue-colored compound. |
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Enzyme Power
Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you'll break up the DNA, making it harder to see. Use meat tenderizer for enzymes. If you can't find tenderizer, try using pineapple juice or contact lens cleaning solution. |
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Stringy Stuff
Alcohol is less dense than water, so it floats on top. Since two separate layers are formed, all of the grease and the protein that we broke up in the first two steps and the DNA have to decide: "Hmmm...Which layer should I go to?" This is sort of like looking around the room for the most comfortable seat. Some will choose the couch; others might choose the rocking chair. In this case, the protein and grease parts find the bottom, watery layer the most comfortable place, while the DNA prefers the top, alcohol layer. DNA is a long, stringy molecule that likes to clump together. |
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