DNA Profile Analysis
DNA Profile Analysis

DNA amplification profile analysis (DAF fingerprinting) is a simple and rapid technique for delineating genotypic strains of bacteria, fungi and protozoa. The principle is similar to that of integron-mediated PCR in that primers are used to fragment the target DNA. The fragments are then replicated using polymerase chain reaction (PCR) techniques and the amplification products are visualised using gel electrophoresis.

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DNA Profile Analysis
DNA Profile Analysis
DNA profile analysis is not a simple process. Stringent demands are placed on the accuracy and consistency of forensic evidence so that complex, robust, and reproducible guidelines are necessary to assist the analyst and ensure mistakes are eliminated before a final profile is reported.
Guidelines for DNA Profile
The guidelines used for forensic DNA profile interpretation are formulated by investigation and statistical evaluation of all aspects of the analytical procedure. All the resulting rules, formulas, and thresholds are perfectly suited to programming of “expert systems”—software programs that imitate the human expert in decision-based processes to formulate a conclusion.
Expert Systems in Forensic DNA Analysis
Expert systems in forensic DNA analysis will contribute greatly to this field by increasing analytical throughput. The net result of this will be an increase in the human resources available for the research and development of improved methodologies, to ensure that forensic DNA profiling continues to advance at its current impressive rate.
Uncertainty in DNA Profile Analysis
Uncertainty in DNA Profile Analysis
In DNA profile analysis, uncertainty arises due to a number of factors such as sampling error, single bands and correlations within and between loci. One of the most important of these factors is kinship: criminal and innocent suspect may share one or more bands through identity by descent from a common ancestor.
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