Human Tissue Profiling Stem Cell and Pathology Lab Tissue Identification Service

Bio-Synthesis, Inc. (BSI) offers fast and reliable Human Tissue Identification services using STR DNA typing to assist pathologist and clinical laboratories in confirming tissue sample verification and to eliminate possible contamination or misidentification of a patient/ donor sample. DNA profiling by short tandem repeats (STR or microsatellite markers) is a fast, affordable and highly discriminating method to identify human tissue. Due to the highly polymorphic nature of each STR locus, every human tissue will have a unique digital bar code that can distinguish it from others. Our facility is equipped with state-of-art technology by providing client with Human Tissue Identification services with speed and reliability.

Highly Informative
Utilizes multiplex STR technology, which includes analysis of 15 genetic loci and 1 sex determination locus (Amelogenin) for high resolution profiling.
Highly Sensitive
Capable of positively matching unknown and reference patient/donor samples to 99.9999+% certainty.
High Throughput
Based on 5-dye chemistry, which allows higher density multiplexing with better spectral resolution to minimize false positive calls due to fluorescent artifacts.
Integrated Workflow
Utilizes Applied Biosystems Genetic Analyzer, GeneScan, GeneMapper ® ID software.
Fast Turnaround
5-7 business days

Call our DNA specialists at 800-DNA-EXAM (800-362-3926)/888-786-9323 for details.

Applications for STR typing in human identity testing and DNA diagnostics

Forensic DNA Casework
DNA Databases
Mass Disasters
Missing Persons
Military Casualties
Parentage and Kinship Testing: paternity testing, immigration issues, inheritance claims
Genetic Genealogy & Answering Historical Questions
Authenticating Human Cell Lines
Detecting Genetic Chimeras
Monitoring Transplants
Monitoring Needle Sharing
Detecting Cancer Tumors
Mapping Genetic Diseases
Examining Human Population Diversity

The Solution Through Use of STR DNA Typing
The DNA typing process used at Bio-Synthesis involves simultaneously amplifying fifteen STR loci and the Amelogenin locus in a multiplex PCR reaction (from Applied Biosystems AmpFlSTR® Identifiler) capable of detecting as low as 500 picograms of genomic DNA (~ 15 diploid cells). The amplicons are separated by capillary electrophoresis and analyzed using Applied Biosystems Identifiler kit and GeneMapper software. The pattern of repeat results in a unique STR identity profile for known (reference sample) or unknown sample. The unknown and reference sample profiles can be used for comparison analysis. The two profiles should result in an exact match of greater than 99.99+% certainties. If the profiles do not match, then it can be determined that an error occurred with the placement or labeling of the unknown sample when it was first collected. Further testing can be performed on other known patient/donor samples to identify the unknown sample.